rat skin fibroblasts Search Results


rat skin fibroblasts rs1  (Keygen Biotech)
86
Keygen Biotech rat skin fibroblasts rs1
LTCONS_00014107 downregulation was screened from NMBA-induced precancerous lesions of heat-stimulated rat esophageal mucosa. ( A ) Schematic diagram of animal experiment protocol. ( B ) Flow chart of differential expression gene screening and the number of differentially expressed lncRNA induced by heat stimulation. Known LncRNA is what has been reported; Novel LncRNA is unknown LncRNA. ( C ) The expression level of novel LncRNAs induced by heat stimulation were showed in heat map. ( D ) The expression level of LTCONS_00014107 was detected in rat esophageal tissues. ( E ) qRT-PCR was used to verify the expression level of LTCONS_00014107 in heat-stimulated <t>RS1</t> cells. ** P < 0.01
Rat Skin Fibroblasts Rs1, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+skin+fibroblasts/pmc12619460-57-0-8?v=Keygen+Biotech
Average 86 stars, based on 1 article reviews
rat skin fibroblasts rs1 - by Bioz Stars, 2026-08
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rat skin fibroblasts  (AcceGen Biotechnology)
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Rat skin fibroblasts, 2-week Wistar rat
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LTCONS_00014107 downregulation was screened from NMBA-induced precancerous lesions of heat-stimulated rat esophageal mucosa. ( A ) Schematic diagram of animal experiment protocol. ( B ) Flow chart of differential expression gene screening and the number of differentially expressed lncRNA induced by heat stimulation. Known LncRNA is what has been reported; Novel LncRNA is unknown LncRNA. ( C ) The expression level of novel LncRNAs induced by heat stimulation were showed in heat map. ( D ) The expression level of LTCONS_00014107 was detected in rat esophageal tissues. ( E ) qRT-PCR was used to verify the expression level of LTCONS_00014107 in heat-stimulated RS1 cells. ** P < 0.01

Journal: Cancer Cell International

Article Title: LTCONS_00014107-miR-29b-2-3p-GPR37 axis promotes heat stimulation induced esophageal carcinogenesis

doi: 10.1186/s12935-025-04041-0

Figure Lengend Snippet: LTCONS_00014107 downregulation was screened from NMBA-induced precancerous lesions of heat-stimulated rat esophageal mucosa. ( A ) Schematic diagram of animal experiment protocol. ( B ) Flow chart of differential expression gene screening and the number of differentially expressed lncRNA induced by heat stimulation. Known LncRNA is what has been reported; Novel LncRNA is unknown LncRNA. ( C ) The expression level of novel LncRNAs induced by heat stimulation were showed in heat map. ( D ) The expression level of LTCONS_00014107 was detected in rat esophageal tissues. ( E ) qRT-PCR was used to verify the expression level of LTCONS_00014107 in heat-stimulated RS1 cells. ** P < 0.01

Article Snippet: Rat skin fibroblasts RS1 were purchased from Jiangsu KeyGEN BioTECH Corp.Ltd.

Techniques: Quantitative Proteomics, Expressing, Quantitative RT-PCR

Knockdown LTCONS_00014107 can inhibit cell growth in RS1 cells and miR-29b-2-3p is up-regulated in rat tissues. ( A ) The position of LTCONS_00014107 sequence where the 3 pairs of siRNA were positioned. ( B ) qRT-PCR was used to verify the knockdown efficiency of LTCONS_00014107 in RS1 cells. ( C ) MTT assay (MTT, 3 mg/ml) and ( D ) soft agar colony formation assay were used to detect the effect of LTCONS_00014107 knockdown on RS1 cell proliferation. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Cancer Cell International

Article Title: LTCONS_00014107-miR-29b-2-3p-GPR37 axis promotes heat stimulation induced esophageal carcinogenesis

doi: 10.1186/s12935-025-04041-0

Figure Lengend Snippet: Knockdown LTCONS_00014107 can inhibit cell growth in RS1 cells and miR-29b-2-3p is up-regulated in rat tissues. ( A ) The position of LTCONS_00014107 sequence where the 3 pairs of siRNA were positioned. ( B ) qRT-PCR was used to verify the knockdown efficiency of LTCONS_00014107 in RS1 cells. ( C ) MTT assay (MTT, 3 mg/ml) and ( D ) soft agar colony formation assay were used to detect the effect of LTCONS_00014107 knockdown on RS1 cell proliferation. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Rat skin fibroblasts RS1 were purchased from Jiangsu KeyGEN BioTECH Corp.Ltd.

Techniques: Knockdown, Sequencing, Quantitative RT-PCR, MTT Assay, Soft Agar Assay

LTCONS_00014107 may be the precursor of miR-29b-2-3p and miR-29b-2-3p is up-regulated in ESCC. ( A ) The expression level of miR-29b-2-3p was detected in rat esophageal tissues. ( B ) qRT-PCR was used to verify the expression level of miR-29b-2-3p in heat-stimulated RS1 cells. ( C ) Degradation of LTCONS_00014107 led to upregulation of miR-29b-2-3p. ( D ) qRT-PCR was used to verify the expression level of miR-29b-2-3p in LTCONS_00014107 knockdown cells. ( E ) RS1 cell activity was detected by MTT assay (MTT, 3 mg/ml). ( F ) miR-29b-2-3p has sequence homology among different species. ( G ) miR-29b-2-3p expression levels were checked in SHEE cells, which were treated at 42℃ water for 1 h and then cultured at 37℃ for 1 h, 2 h, 4–6 h. ( H ) The expression of miR-29b-2-3p in ESCC cell lines. (I) Higher expression of miR-29b-2-3p was associated with lower survival. (J) The expression of miR-29b-2-3p was detected by ISH in normal and preneoplastic lesions of human esophagus tissues. The positive staining was counted. Data are all shown as mean ± S.D. from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Cancer Cell International

Article Title: LTCONS_00014107-miR-29b-2-3p-GPR37 axis promotes heat stimulation induced esophageal carcinogenesis

doi: 10.1186/s12935-025-04041-0

Figure Lengend Snippet: LTCONS_00014107 may be the precursor of miR-29b-2-3p and miR-29b-2-3p is up-regulated in ESCC. ( A ) The expression level of miR-29b-2-3p was detected in rat esophageal tissues. ( B ) qRT-PCR was used to verify the expression level of miR-29b-2-3p in heat-stimulated RS1 cells. ( C ) Degradation of LTCONS_00014107 led to upregulation of miR-29b-2-3p. ( D ) qRT-PCR was used to verify the expression level of miR-29b-2-3p in LTCONS_00014107 knockdown cells. ( E ) RS1 cell activity was detected by MTT assay (MTT, 3 mg/ml). ( F ) miR-29b-2-3p has sequence homology among different species. ( G ) miR-29b-2-3p expression levels were checked in SHEE cells, which were treated at 42℃ water for 1 h and then cultured at 37℃ for 1 h, 2 h, 4–6 h. ( H ) The expression of miR-29b-2-3p in ESCC cell lines. (I) Higher expression of miR-29b-2-3p was associated with lower survival. (J) The expression of miR-29b-2-3p was detected by ISH in normal and preneoplastic lesions of human esophagus tissues. The positive staining was counted. Data are all shown as mean ± S.D. from three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: Rat skin fibroblasts RS1 were purchased from Jiangsu KeyGEN BioTECH Corp.Ltd.

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Activity Assay, MTT Assay, Sequencing, Cell Culture, Staining